Ray Gun is MiraMate’s next-generation VUV light device, designed to treat many kinds of surface and deep skin issues more effectively and safely. No other device can do what Ray Gun can do; allow the effects of VUV light to penetrate skin to kill pathogens without damaging healthy cells. In this experiment, we tested the effectiveness of Ray Gun on Human melanoma. We used CCK8 to assay the effect on the invitro proliferative activity of A-375 cells.
Ray Gun, human malignant melanoma cancer cell A-375, BALB/c nude mice, DMEM medium, fetal calf serum, PBS, CCK-8 kit
The skin used in this test was harvested from mice euthanized for other lab tests. (No animal was hurt or killed for the purpose of this test.)
Arc mode is when the tube is 1-2mm away from the treatment surface.
Experimental Process and Results
Step 1: Mouse sterile skin preparation.
BALB/c nude mice were completely immersed in 75% ethanol and soaked for 15-20 min.
The mice were then placed in an ultra-clean table, and a circular portion of skin between the armpit and groin was removed, washed with sterile PBS, and placed aside for later use.
Step 2: Measure the logarithmic growth phase of the cancer cells. A 1×105 cells/mL suspension was made. This was then seeded in a 6-well plate (2 mL/well), and placed in a 37 ℃, 5% CO2 incubator for 24 hours.
Step 3: Divide the suspension samples into 5 groups.
The samples were divided into 5 groups. Each group had differing treatment methods. One group (the ‘control’) was left untreated. The cell morphology of each group was observed and photographed under the microscope at 0 h, 24 h and 48 h time periods.
Step 4: Incubation.
100 μL of CCK-8 reagent added to each well, and incubated at 37°C for 2-4 hours without light.
Measure the absorbance at 450 nm, and calculate the proliferation inhibition rate.
Step 5: Compare the value of absorbance and proliferation inhibition rate in each group after 24 and 48 hours.
Result at 0h
Result at 24h:
Result at 48h:
There was no significant effect on the invitro proliferative activity of cells in each group immediately after Ray Gun treatment.
After 24 hours, Arc mode VUV with earthing, no skin (item 5) significantly inhibited cell proliferation activity and apoptosis by 33.36% compared with the control group (item 1).
After the lamp was wrapped around the skin (Item 3: Contact mode VUV with earthing and skin.), the inhibitory effect of the device on cell proliferation was reduced (though still significant). The apoptosis rate was 16.49%. No significant effect was seen in the other groups.
After 48 h, the inhibitory effect of the Ray Gun on groups 3 and 5 increased further, with 39.92% and 73.63% inhibition rates respectively. In addition, Ray Gun had a greater inhibitory effect on item 4 (Arc mode VUV with earthing and skin. The cells also displayed significant proliferation inhibition, with an inhibition rate of 12.91%. No significant effect of Ray Gun on the cells of the item 2 (Contact mode VUV with skin) group was observed during the experiment.
According to the experiment results, Ray Gun is lethal to human malignant melanoma cells, and can significantly inhibit human melanoma cell proliferation activity, particularly when used with earthing.